The Basic Principles Of attune flow cytometer
The Basic Principles Of attune flow cytometer
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Knowledge-enriched characterization of cell populations. Morphological information and facts from illustrations or photos can add into the richness of flow cytometry knowledge. One example is, the determine shows an usually typical apoptosis assay using Annexin V and PI, introducing cell imaging to characterize cells in Each and every inhabitants to reveal morphologically distinctive attributes. These insights couldn't are already obtained from multiplex staining on your own.
As samples are acquired on the Attune CytPix Flow Cytometer, the significant-pace brightfield digital camera captures and shops visuals of detected occasions in a charge around 6,000 images per next, with regards to the flow level and graphic measurement. For bigger versatility, Attune Cytometric Software package lets you modify the impression capture frequency as wanted and select a certain gate for graphic seize, if necessary.
(B) Uncooked 264.seven macrophages are sometimes annotated inaccurately with respect to particle depend due to their complexity and noncircular borders. The number of singlets obtainable for Assessment increases when the person trains and employs a more correct design.
Find new insights into your experimental facts by visualizing populations using mixed fluorescence and morphology info. Our computer software interface wizards enable downsampling, concatenation (merged documents) and dimensionality equipment that are designed to the software package for an intuitive, streamlined facts Investigation practical experience.
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Furthermore, it can be illuminating to view the amount of doublets slip by traditional singlet gates, and good to verify the status of unforeseen double-constructive gatherings in my Investigation."
Optimizing gating tactic. Even sturdy manual singlet gating is error-vulnerable and continues to be a subjective final decision stage in Pretty much all flow cytometry assays. Imaging may be used to substantiate and adjust gates to incorporate only single cells of desire.
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The Attune NxT cytometer is the reliable workhorse instrument your lab can rely upon for constant and trustworthy overall performance. The Attune CytPix model contains a superior-speed brightfield digital camera and AI-pushed image analysis computer software, with the latest updates which includes equipment Mastering resources and an intuitive user interface, enabling Improved knowledge precision, accuracy, and more quickly insights into sample biology.
Customers can watch occasion images in authentic-time to verify sample high quality, enhance protocols, and record the morphology of every mobile population determined for Investigation. Imaging seize also Added benefits from acoustic concentrating, which really helps to place the cells to ensure a sharp, centered picture is acquired.
AI-driven automatic image Examination Together with the Attune Cytometric Program translates celebration characteristics into distinctive morphology parameters which might be combined with regular fluorescence and scatter parameters to reveal highly reproducible inhabitants improvements and exceptional subsets.
We Formerly demonstrated the strength of imaging Automobile-T/Ramos mobile interactions. Let’s have a look at just the inhabitants of biggest curiosity, the double optimistic functions, to learn more. We can easily now use prolonged morphological parameters (in this article, circularity and skewness of depth) to more take a look at the attributes of those populations and refine gating on these gatherings, increasing information robustness.
Built-in dimensionality reduction tools elevated resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to verify anticipated inhabitants resolution in lysed entire human blood. Consumer can see events from Every cluster for Visible confirmation of cell phenotypes.
Flexible payment approach from Agilent Fiscal Options assist you to obtain the important instrumentation you need for immediate job, or to expand your lab.
At the guts of each and every Attune Flow Cytometer lies our innovative acoustic focusing know-how, which utilizes seem waves to meticulously align cells in the flow mobile. This progressive technique surpasses classic hydrodynamic techniques, leading to more quickly processing here speeds, exceptional knowledge good quality, and increased sample throughput.
As the cell line experienced Earlier been analyzed on the Attune CytPix Flow Cytometer, the researchers went back to the pictures and were being ready to document the microbial an infection a minimum of 5 times before.
Agilent Price Assure ensures you a minimum of ten years of instrument use within the day you purchase. Or else, We'll credit history you Along with the residual worth of the method toward an upgraded product.
What seemed to be a homogeneous inhabitants is definitely extra numerous—an Perception that ought to be deemed when interpreting effects.